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Work Background
University of Wisconsin-MadisonGraduate Research Assistant
Aug. 2018Madison, Wisconsin, United StatesWeeks Lab • Protein engineering of an enzyme for LC-MS proteomics analysis of phosphorylation events in living cells.
• Protein engineering, expression, purification. • Mammalian cell cultures, lentiviral expression system, immunostaining.
• Peptide synthesis, HPLC purification, MS analysis.
• Generation and LC-MS proteomic analysis (with the use of self-developed python scripts) of bacterial and mammalian peptide libraries (including phospho-enriched libraries).
The University of ChicagoResearch Assistant
Sep. 2016 - Jul. 2018Greater Chicago AreaKossiakoff Lab • Development of synthetic affinity reagents based on immunoglobulin and immunoglobulin binding protein scaffold as crystallization chaperones and fiducial markers for single particle cryoEM. • Generation of synthetic antibodies of soluble and membrane proteins and multiprotein complexes using phage display • Protein and antibody engineering, expression, purification, crystallization. • Mammalian and insect cell cultures, antibody labeling, cell viability assays. • Characterization of antigen-antibody complexes using DSF, SPR, ELISA
The University of ChicagoResearch Assistant
Jul. 2015 - Sep. 2016Greater Chicago Area
Institute of Immunology and Experimental Therapy, Polish Academy of SciencesIntern
Sep. 2013 - Jan. 2015Wrocław Area, PolandDevelopment of new drug delivery systems: synthesis, purification and physicochemical
characterization of polymer – drug conjugates.
Establishing new biophysical methods for studying stability of drug delivery systems
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