Dario Leone

Field application scientist @Miltenyi

Tutor💸 MarketingResearcherProfessor🧮 EducationLecturerScientist

Pre-sale and post-sale support on the filed for flow-cytometry products and instrument present in the Miltenyi portfolio that include MACSQuant 10/16/VYB and Tyto cell sorter. Quality control and Quality assurance of all clinical procedure performed on the CliniMACS Prodigy including; CD34 enrichment, alfa/beta TCR T-cell depletion, generation of CAR-T in pre-clinical and clinical stage, Virus specific T-cell isolation and expansion and other. Capable of building a relationship with important customer (business development) and follow their projects in tailored application and cutting-edge cell therapeutic projects. My list of client goes from hospital like Bambin Gesú pediatric hospital (OPBG) either for cell manufactoring process (R. Bugianesi), the Department of Pediatric Onco-Hematology and Transfusion Medicine (G. Li Pira), Institute Romagnolo for the tumor study Dino Amadori (Meldola), the Mediterranean Institute for Transplantation (ISMETT, Palermo), the Giglio Hospital (Cefalù) but also other company like GSK vaccines (Siena), Evotec (Medolla, Verona) and others. Marketing activity | Speaker in conferences (GIC, ISCCA, Brayn, ACC and other), training, organization of meeting from basic to advanced, business development discussion.

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Work Background

Miltenyi BiotecFlow cytometey and imaging specialist

Mar. 2020Bologna, Emilia Romagna, Italia

Miltenyi BiotecFlow cytometry specialist

Mar. 2020Bologna, Italia

CIMLPostdoctoral Research Fellow

Mar. 2018 - Mar. 2020Marseille Area, FranceRole of BAD-LAMP in IFN-a production by plasmacytoid Dendritic cell

CIMLPostdoctoral Research Fellow

Mar. 2018 - Mar. 2020Marseille Area, France

MUW Medical University of ViennaPost-doc Clinical Institute of Pathology

Jul. 2016 - Feb. 2018Vienna

MUW Medical University of WienPhd in Immunology

Sep. 2010 - Apr. 2016Wien, AustriaProject title1 founded by Marie Curie Action (ESR) TRanSvir network: Circulating LAMP-2 Autoantibody in Focal Necrotizing Glomerulo Nephritis (FNGN) patient Interact with Human Monocytes Derived Dendritic cell (MDDC) modulating the balance between tolerance and immunity influencing their antigen presentation capability. Principal subjects/skills covered: In vitro generation of MDDC from peripheral blood. Pulsing of MDDC with monoclonal or polyclonal anti-Lamp-2 antibody and ideally with autoantibody isolated from patients serum. In-vitro antigen presentation study to identify mechanisms causing breaking of tolerance against MPO and Pr3. Project title2: Characterization of Mononuclear Phagocytic System (MPS) in human kidney. Principal subjects/skills covered: Flow-cytometry and histology are use to classify subpopulation of monocytes, dendritic cell and macrophages that are present in donor or nephrectomy kidney specimen.

NKI-AVLResearch Technician

Feb. 2009 - Jul. 2009Principal techniques and skills: DC isolation from mouse spleen, bone marrow DC in vitro differentiation, FACS staining and analysis, long-term stromal cell culture for production of recombinant protein, protein purification, immunofluorescence staining and analysis, long term imaging with wide field microscopy system (BD pathway 855 system and integrated software including programming macro script for 5 days in-vivo imaging). Animal skill: Bone marrow and spleen isolation, IP and IV injection, blood collection from tail, X ray treatment.