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Work Background
Miltenyi BiotecFlow cytometey and imaging specialist
Mar. 2020Bologna, Emilia Romagna, Italia
Miltenyi BiotecFlow cytometry specialist
Mar. 2020Bologna, Italia
CIMLPostdoctoral Research Fellow
Mar. 2018 - Mar. 2020Marseille Area, FranceRole of BAD-LAMP in IFN-a production by plasmacytoid Dendritic cell
CIMLPostdoctoral Research Fellow
Mar. 2018 - Mar. 2020Marseille Area, France
MUW Medical University of ViennaPost-doc Clinical Institute of Pathology
Jul. 2016 - Feb. 2018Vienna
MUW Medical University of WienPhd in Immunology
Sep. 2010 - Apr. 2016Wien, AustriaProject title1 founded by Marie Curie Action (ESR) TRanSvir network:
Circulating LAMP-2 Autoantibody in Focal Necrotizing Glomerulo Nephritis (FNGN) patient Interact with Human Monocytes Derived Dendritic cell (MDDC) modulating the balance between tolerance and immunity influencing their antigen presentation capability. Principal subjects/skills covered:
In vitro generation of MDDC from peripheral blood. Pulsing of MDDC with monoclonal or polyclonal anti-Lamp-2 antibody and ideally with autoantibody isolated from patients serum. In-vitro antigen presentation study to identify mechanisms causing breaking of tolerance against MPO and Pr3. Project title2: Characterization of Mononuclear Phagocytic System (MPS) in human kidney. Principal subjects/skills covered:
Flow-cytometry and histology are use to classify subpopulation of monocytes, dendritic cell and macrophages that are present in donor or nephrectomy kidney specimen.
NKI-AVLResearch Technician
Feb. 2009 - Jul. 2009Principal techniques and skills:
DC isolation from mouse spleen, bone marrow DC in vitro differentiation, FACS staining and analysis, long-term stromal cell culture for production of recombinant protein, protein purification, immunofluorescence staining and analysis, long term imaging with wide field microscopy system (BD pathway 855 system and integrated software including programming macro script for 5 days in-vivo imaging).
Animal skill: Bone marrow and spleen isolation, IP and IV injection, blood collection from tail, X ray treatment.
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