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Work Background
North West London PathologyAssociate Practitioner
Dec. 2025London Area, United Kingdom
Barts Health NHS TrustAssociate Practitioner (Locum)
Nov. 2025 - Nov. 2025London Area, United Kingdom
Biomed Laboratory LtdMolecular Biology Intern
Apr. 2025 - Sep. 2025Milton Keynes, England, United Kingdom- Cell Culture; Using MCF-7 cell line for human breast cancer, this enabled a better understanding of how cell culture should be conducted by growing human cells in a suspension comprised of media, within a controlled, artificial, in vitro environment. Cell growth was monitored whilst adhering to aseptic technique in order to reduce the risk of contamination
- Cell Passaging; Subculturing cells by moving some cells to a fresh flask with media, in order to maintain optimal growth and prevent overconfluence
- Cell Counting; Using a hemocytometer in order to calculate the cell concentration and then calculating the desired volume of media or cell concentration for the next cell culture, using these values
- DNA Extraction; Helping procure genetic material (i.e. DNA) needed for PCR
- PCR Amplification; Using template DNA, millions of DNA copies can be created using a thermal cycler
- Gel Electrophoresis; Used to visualise DNA fragments by separation based on size, allowing us to check whether PCR has worked
- Nanodrop spectrophotometry; Equipment used to measure the concentration & purity of nucleic acids and proteins
- In silico primer guide design; software used to help create primers, used in CRISPR
- Western Blotting
St George's University Hospitals NHS Foundation TrustMedical Laboratory Assistant (Locum)
Nov. 2024 - May. 2025London- Slide filing and archiving
- Case assembly; completing cases by block checking against slides, with the the accompanying specimen
- Specimen Reception; labelling request forms with unique laboratory numbers, relevant specialty and specimen code, along with the accompanying specimen
- Specimen Triaging; differentiating specimens based on whether they are either routine or urgent biopsies; or routine or urgent 'hotseat' (slightly larger specimens, that can be bisected)
- Dissection assistance; printing cassettes to be used for specimen processing, inputting patient information onto the laboratory information management systems (i.e. WinPath and Cerebro) and creating new dictations via G2 Speech
- Loading blocks for pegasus processing - either running routine or fatty programs, depending on the loaded basket (e.g. routine, fatty, biopsy)
- Dealing with leaking samples, leaving them to dry on a downflow bench
- Helping set up and eventually clean up specimen dissection
- Laboratory and General Maintenance, along with Stock Upkeep (e.g. PPE, cassettes, aprons, sleeve protectors, formalin pads, specimen pots)
Biomed Laboratory LimitedHistopathology Intern
Mar. 2024 - Nov. 2024Milton Keynes, England, United Kingdom- Specimen Reception; recording specimen into the laboratory information management system (LIMS) and checking the details on the request form match
- Specimen Dissection Assistance; helping dissect specimens into small samples, ready for tissue processing
- Embedding; placing specimens into a paraffin wax block, ready for microtomy
- Microtomy; creating sections using a microtome to cut the blocks, suitable to be placed on a glass slide
- Haemotoxylin & Eosin (H&E) Staining
- Special Staining (e.g. Miller’s Stain, Periodic Acid Schiff (PAS), Martius Scarlet Blue (MSB), Alcian Blue); utilised when attempting to identify particular elements that couldn’t be distinguished using standard H&E staining
- Coverslipping glass slides, so they’re ready to be observed under the microscope and for quality control - Quality Control; check the ready slide under the microscope to ensure everything has been done correctly and comparing this against the wax block the sample was procured from, as well as checking for any potential errors or mistakes
- Maintaining cleanliness whilst in the laboratory, along with correctly following procedures
King's College LondonGraduate Research Assistant
Jul. 2023 - Jan. 2024London, England, United KingdomAssisting Dr Vasco Claro, PhD, in his research which has been looking at the interplay of netrin-1 and THP-1 monocytes in the progression of atherosclerosis, along with how they may influence this. My duties within the role included:
- Performing tissue culture,typically 2-3 times a week; feeding cells with specific media (RPMI & FBS) to aid in cell growth
- Monitoring the cells to observe any potential signs of infection and other issues that may arise
- Recording cell count, using a hemocytometer and loading cells properly into the hemocytometer
- Performing scientific calculations to determine exact measurements of cell culture and media required, when creating a new cell culture
- Creating new cell cultures/dilutions
- Differentiation of THP-1 monocytes into macrophages
- Observing and understanding cell freezing
- Observing, assisting and understanding mRNA isolation, which was required to produce cDNA for PCR (Polymerase Chain Reaction)
- Independent reading of research papers, helping solify my understanding of the research being undertaken
- Good Laboratory Practice (GLP) (i.e. cleaning safety cabinet used, wearing gloves when handling lab equipment, storing equipment & chemicals in the correct place in the lab, handling lab equipment that I have been trained to use)
- Proper, correct use of laboratory equipment Additional skills:
- Use of a confocal microscope
- Observing and understanding gel electrophoresis
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