University of North Dakota School of Medicine and Health SciencesPhD, Biomedical Sciences
Aug. 2015 - Jun. 2021United States of AmericaUsed: - CRISPR/Cas9 to knock out gene of interest - Cell based assays ( migration, proliferation, angiogenesis) - Protein assays (BCA, ELISA, IF, western blotting) - Molecular biology (DNA/RNA extraction, PCR, Sanger sequencing, RNA seq, plasmid construction, transfection, Lentiviral transductions, bacterial culture) - Immunofluorescence microscopy o Live cell imaging (DMi8 Leica Thunder) o Confocal imaging (Olympus FV1000 MPE, Olympus FV3000) o Epifluorescence imaging (DMi8 Leica Thunder) o Calcium imaging (Olympus IX83) o Fluorescence Recovery After Photobleaching (FRAP) (Zeiss LSM-510) -Bio-image informatics o Designed workflows for multi-parametric analysis of cellular images. o Used FIJI/Image J macros for batch processing of images. o Used FIJI/Image J plugins to quantify data from images in an unbiased manner. - Bioinformatics - oRNA- seq analysis of CRISPR/Cas9 mediated knock out cell lines to identify protein variants, differentially expressed genes and off targets. - Mass Spectrometry - o Extraction of lipid oxidation products from pulverised fish samples. o Prepared samples and ran on UPLC – MS (Xevo TQ‐S, Waters) - Managed laboratory - Teaching Assistant ( Microbiology 2020- undergraduates & Methods for quantitative imaging - graduates) - Tutored Block I biochemistry for InMed program - medical students -Statistical Analysis using GraphPad Prism – Power Analysis, Student’s T Test, One- and Two-way ANOVA with multiple comparisons with post hoc analysis as corrections for multiple comparison. -Maintained laboratory operations, updating and revising standardised operating procedures and reordering raw materials and supplies to sustain experiments and workflow. -Managed and trained 4 students in laboratory.